Identification of EnterohemorrhagicEscherichia coliO157-Specific DNA Sequence Obtained from Amplified Fragment Length Polymorphism Analysis
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چکیده
منابع مشابه
DNA Extraction Strategies for Amplified Fragment Length Polymorphism Analysis
A polymerase chain reaction-based DNA typing method, amplified fragment length polymorphism (AMP-FLP) analysis, has shown promise as a means of analyzing forensic biological evidence. A variety of DNA extraction methods were evaluated for their suitability for AMP-FLP analysis. Factors that were considered in the evaluation included DNA yield, ability of DNA to be amplified, the presence of DNA...
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An amplified fragment length polymorphism (AFLP) method was applied to 129 strains representing 24 different Clostridium species, with special emphasis on pathogenic clostridia of medical or veterinary interest, to assess the potential of AFLP for identification of clostridia. In addition, the ability of the same AFLP protocol to type clostridia at the strain level was assessed by focusing on C...
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Amplified fragment length polymorphism (AFLP) analysis of botanical forensic evidence provides a means of obtaining a reproducible DNA profile in a relatively short period of time in species for which no sequence information is available. AFLP profiles were obtained for 40 Acer rubrum trees. Leaf material from five additional species was also typed. Genomic DNA was isolated using the DNeasy Pla...
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P. H. M. SAVELKOUL,* H. J. M. AARTS, J. DE HAAS, L. DIJKSHOORN, B. DUIM, M. OTSEN, J. L. W. RADEMAKER, L. SCHOULS, AND J. A. LENSTRA Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, Amsterdam, DLO-State Institute of Quality Control of Agricultural Products Centre De Born, Wageningen, Novartis Seeds bv, Section Biotechnology, Enkhuizen, Department...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 2007
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.2007.tb03970.x